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IGVFFI0944TCGC

released
validated
File Set
IGVFDS7994GDON(gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in many genes)
Summary
R2 reads from sequencing run 4
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:hPA-Sequence-File-perturbseq-10xlane1-CRISPR-L1-R2
md5sum
5a0e163f13d3daa1883fb1e634ff0aad
Content MD5sum
72eab207e59ab62161bb3b78e089646f
File Size
1.5 GB
Submitted File Name
/work/rr151/SamAIM2neuro_data/CRISPR_1_S1_L001_R2_001.fastq.gz
Checkfiles Version
v20
Attribution
Charles Gersbach, Duke

Sequencing Details

Sequencing Platform
Illumina NovaSeq 6000
Sequencing Run
4
Lane
1
Sequencing Kit
NovaSeq 6000 S2 Reagent Kit v1.5
Illumina Read Type
R2
Average Read Length
90
Read Count
48650333

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
Homo sapiens astrocyte cell treated with 10 ng/mL Neurotrophin-3, 20 ng/mL Brain-Derived Neurotrophic Factor, modified with CRISPRa Sp-dCas9-2xVP64-2A-Puro, transduced (lentivirus) with a guide library

Associated seqspec Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
yaml
seqspec
seqspec of IGVFFI0515QEWR, IGVFFI0944TCGC
Charles Gersbach, Duke
1.4 KB
validated