IGVFFI0937NRCQ

preview

validated

This object is in preview status. Metadata is subject to change until this object is released.

File Set

IGVFDS9847QETL(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

R1 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep3-BinC-PCRRep2_R1

md5sum

cdf9bec0a179316a47a86877f0e18dfc

Content MD5sum

2a9fb129e359be27433e2eadeb09d838

File Size

6.1 MB

Submitted File Name

Jurkat_Stim-IL2RA_prom-BioRep1-FFRep3-PCRRep2-BinC_S35_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NovaSeq X

Sequencing Run

Illumina Read Type

Index

GACCTAAC:TTGT

Average Read Length

150

Read Count

206556

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1409FVBR

InVitroSystem

Jurkat

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library