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IGVFFI0921RODS

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS2239WKRH(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:240908-S029-PPIF_5SpliceSitePool-THP1-BioRep2-FFrep1-BinA-PCRrep4_I2
md5sum
daf454a74b22a9cf137925586b74d612
Content MD5sum
e4130021904bcbfc5685baf5aa023548
File Size
1.3 MB
Submitted File Name
240908-S029-5splice-THP1-5splicesite_pool-Amp_PPIF_5splicesite-BioRep2-FFrep1-BinA-PCRrep4_S751_I2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NovaSeq X
Sequencing Run
1
Illumina Read Type
I2
Index
TGTAATCA:GATATCGA
Average Read Length
8
Read Count
167303

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
variant effects
variant effects
Jesse Engreitz, Stanford
pending

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford