IGVFFI0872SIOH

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS9444MPCG(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep1-BinA-PCRRep7_I1
md5sum
884d9944b5e90dcbe14c00dfc1da8ef0
Content MD5sum
5c786b3d597120d0838d5974dcef44f2
File Size
2.3 MB
Submitted File Name
Jurkat_Stim-IL2RA_prom-BioRep2-FFRep1-PCRRep7-BinA_S157_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Illumina Read Type
I1
Index
AGTTGCTT:GGCA
Average Read Length
8
Read Count
247614

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
variant effects
variant effects
Jesse Engreitz, Stanford
5.9 KB
validated

Attribution