IGVFFI0840FMQW

released

validated

File Set

IGVFDS3611WVHW(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF1-BinE-PCR2-Rep1_S119

md5sum

d22e07c738037d6e44947c0e5a632c95

Content MD5sum

4e4cd20c97a1790382524887f1a6f45c

File Size

25 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF1-BinE-PCR2-Rep1_S119_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CCTACCAT:CCAG

Average Read Length

Read Count

1145823

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4222TCYN

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library