IGVFFI0835ASTY

released

validated

File Set

IGVFDS6731AKUS(unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

R2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep1-FFrep2-FACS_Input-PCRrep2_R2

md5sum

8e135a8961c9a155b1c6c5bb58d8864e

Content MD5sum

633787dafcf660a70fb53d04d806475c

File Size

75 MB

Submitted File Name

220228pPPIF-FFSample-3-D21-pPPIF-Amp-pPPIF-1-2-2-BinAll_R2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NextSeq 550

Sequencing Run

1

Illumina Read Type

R2

Index

CACATCCT:TGAC

Average Read Length

150

Read Count

1910970

Samples

1 item

Accession

Type

Sample Terms

Summary

InVitroSystem

THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award

Principal Investigator(s)

Anshul Kundaje, Jesse Engreitz, Marlene Rabinovitch, Lars Steinmetz, Michael Bassik, Thomas Quertermous, Will Greenleaf

Contact P.I.

Lab

Jesse Engreitz, Stanford