IGVFFI0805QHNX

released

validated

File Set

IGVFDS4662ZYNK(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF4-BinD-PCR2_S46

md5sum

a0ec2b280dc2772d323e02d39a49cb52

Content MD5sum

84479670979658b54d965de51b0e238a

File Size

7.8 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF4-BinD-PCR2_S46_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ATTGTCTG:CTCT

Average Read Length

Read Count

323593

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1961BHPA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library