IGVFFI0797YDWG

released
validated
File Set
IGVFDS6756WKPU(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 2
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep4-FFrep1-BinA-PCRrep1_I2
md5sum
110bb619c887e1ff681237cb834748a8
Content MD5sum
a16e7eba220f1ceb5d860c12415c8c7d
File Size
2.6 MB
Submitted File Name
220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep2-FFRep1-BinA-PCRrep1_S22_I2_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
2
Illumina Read Type
I2
Index
ATTCCTCT:ACTG
Average Read Length
4
Read Count
344782

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-10% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution