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IGVFFI0797HGEV

released
validated
File Set
IGVFDS2388VNTG(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep1-BinC-PCRrep12_R1
md5sum
2409b51d9722402d8478865abe0f6560
Content MD5sum
5145d836b7778b4a8ce59faf9f8cb327
File Size
5.5 MB
Submitted File Name
230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep2-FFRep1_BinC-PCRrep12_S228_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
R1
Index
GCAGCCGT:ACTG
Average Read Length
150
Read Count
185343

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford