IGVFFI0790UGVP

released
validated
File Set
IGVFDS3944URNH(gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in B2M)
Summary
R1 reads from sequencing run 2
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:cd8-tcells-measurement-set-crispri-b2m-high-bin-rep2-R1
md5sum
6c479d41090f11ffa24d3ac7c8bd6ea0
Content MD5sum
d61c27d8b2359a724b1b4a07a4161d79
File Size
23 MB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/B2M-High-R2_S8_L001_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
2
Flowcell ID
000000000-J9HPF
Lane
1
Sequencing Kit
MiSeq Reagent Kit v2
Illumina Read Type
R1
Average Read Length
22
Read Count
976457

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
16 MB
validated

Attribution