{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI0747RCCZ/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI0747RCCZ",
"assay_titles": [
"CRISPR FlowFISH screen"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "912fbb9f53b07e30f30d5d4839b3c25c",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-04-16T21:43:12.665081+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS7773OUJT/",
"accession": "IGVFDS7773OUJT",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM3948PYPE/",
"accession": "IGVFSM3948PYPE",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"status": "released",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"status": "released",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"status": "released",
"summary": "gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3"
},
"file_size": 7083212,
"gene_list_for": [],
"href": "/sequence-files/IGVFFI0747RCCZ/@@download/IGVFFI0747RCCZ.fastq.gz",
"illumina_read_type": "I2",
"input_file_for": [],
"integrated_in": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"loci_list_for": [],
"maximum_read_length": 8,
"md5sum": "8b712c60a8547ca23f1424e593189641",
"mean_read_length": 8,
"minimum_read_length": 8,
"read_count": 872981,
"release_timestamp": "2024-06-22T00:27:12.304865+00:00",
"s3_uri": "s3://igvf-files/2024/04/16/0d1c87cd-48cf-4066-8f5c-7a70fc2ffe39/IGVFFI0747RCCZ.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_kit": "MiSeq Reagent Kit v2",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0004205/",
"status": "released",
"term_name": "Illumina MiSeq"
},
"sequencing_run": 1,
"status": "released",
"submitted_by": {
"@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
"title": "Chongyon Park"
},
"submitted_file_name": "/Desktop/NOS3/NOS3_CRISPRi_FlowFISH/231215S023-TeloHAEC-P219-Amp_NOS3-BioRep1-FFrep1-BinC-PCRrep2_S195_I2_001.fastq.gz",
"summary": "I2 reads from sequencing run 1",
"upload_status": "validated",
"uuid": "0d1c87cd-48cf-4066-8f5c-7a70fc2ffe39"
}