IGVFFI0736JJDC

preview

validated

This object is in preview status. Metadata is subject to change until this object is released.

File Set

IGVFDS6967UVNI(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

R1 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep1-BinA-PCRRep4_R1

md5sum

8cd2222a434a6d3484d53f9b73609900

Content MD5sum

75d0b663a4ebc0fd113e748b0e505321

File Size

5.9 MB

Submitted File Name

Jurkat_Stim-IL2RA_prom-BioRep1-FFRep1-PCRRep4-BinA_S73_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NovaSeq X

Sequencing Run

Illumina Read Type

Index

CCAGTTAG:TTGT

Average Read Length

150

Read Count

198099

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9297KXBW

InVitroSystem

Jurkat

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library