IGVFFI0721XHEB

released

validated

File Set

IGVFDS2599ESDV(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin6hrs-FF1-BinA-PCR4-Rep1_S193

md5sum

7c2c944b24d7f68e3c9f30dc2c9f6364

Content MD5sum

2c64f3c9ca93f6e7fd5d43c0688ce471

File Size

34 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin6hrs-FF1-BinA-PCR4-Rep1_S193_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GTCATCTA:ATTG

Average Read Length

Read Count

1555851

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2428RDRI

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library