IGVFFI0713WITI

{
    "@context": "/terms/",
    "@id": "/sequence-files/IGVFFI0713WITI/",
    "@type": [
        "SequenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI0713WITI",
    "aliases": [
        "gary-hon:LW281C_2_R1"
    ],
    "assay_titles": [
        "Perturb-seq"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG011996/",
        "component": "functional characterization"
    },
    "content_md5sum": "d71c2f7d0e30f11ea7aa55bd585cc4e2",
    "content_type": "reads",
    "controlled_access": false,
    "creation_timestamp": "2024-03-01T17:17:46.726464+00:00",
    "externally_hosted": false,
    "file_format": "fastq",
    "file_set": {
        "@id": "/auxiliary-sets/IGVFDS2549GCKW/",
        "accession": "IGVFDS2549GCKW",
        "file_set_type": "cell hashing barcode sequencing",
        "samples": [
            {
                "@id": "/in-vitro-systems/IGVFSM0956HKRJ/",
                "accession": "IGVFSM0956HKRJ",
                "classifications": [
                    "differentiated cell specimen"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
                        "modality": "interference",
                        "status": "preview",
                        "summary": "CRISPRi Sp-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0009747/",
                        "status": "released",
                        "term_name": "GM25256 (WTC-11)"
                    }
                ],
                "status": "preview",
                "summary": "GM25256 (WTC-11) differentiated cell specimen induced to neural stem cell for 20 days (cellular sub pool: subpool_LW281), male, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
                "targeted_sample_term": {
                    "@id": "/sample-terms/CL_0000047/",
                    "status": "preview",
                    "term_name": "neural stem cell"
                },
                "taxa": "Homo sapiens"
            }
        ],
        "status": "preview",
        "summary": "cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
    },
    "file_size": 207256719,
    "flowcell_id": "AAC7VLGHV",
    "gene_list_for": [],
    "href": "/sequence-files/IGVFFI0713WITI/@@download/IGVFFI0713WITI.fastq.gz",
    "illumina_read_type": "R1",
    "input_file_for": [],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/gary-hon/",
        "title": "Gary Hon, UT"
    },
    "lane": 2,
    "loci_list_for": [],
    "maximum_read_length": 28,
    "md5sum": "bd4b6fec94de635611219cbaeaac220b",
    "mean_read_length": 28,
    "minimum_read_length": 28,
    "read_count": 10498328,
    "s3_uri": "s3://igvf-files/2024/03/01/ef6de3f5-2f85-4639-996b-94c3297caab3/IGVFFI0713WITI.fastq.gz",
    "schema_version": "14",
    "seqspecs": [],
    "sequencing_platform": {
        "@id": "/platform-terms/EFO_0010963/",
        "status": "released",
        "term_name": "Illumina NextSeq 2000"
    },
    "sequencing_run": 1,
    "status": "preview",
    "submitted_by": {
        "@id": "/users/b05645f1-2d56-4a5e-b272-4601e555a3fa/",
        "title": "Mpathi Nzima"
    },
    "submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-10-05-McDermott-NextSeq.10X/2023-10-05/outs/fastq_path/AAC7VLGHV/LW281C_S5_L002_R1_001.fastq.gz",
    "summary": "R1 reads from sequencing run 1",
    "upload_status": "validated",
    "uuid": "ef6de3f5-2f85-4639-996b-94c3297caab3"
}