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IGVFFI0713TGNB

released
validated
File Set
IGVFDS6622DPIT(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep2-FFrep1-BinB-PCRrep10_I2
md5sum
e0275fc3bbd298cbb09a84abfd7d25ba
Content MD5sum
2b8d06318291eb4066713cc0d0b3bac9
File Size
1.2 MB
Submitted File Name
230807S022-THP1-P153-Amp_PPIF_promoter-BioRep2-FFrep1-BinB-PCRrep10_S214_I2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I2
Index
ATTCCTCT:ACTG
Average Read Length
4
Read Count
154026

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford