IGVFFI0700SJDG

released

validated

File Set

IGVFDS9738NANI(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinA-PCR4_Rep1_S43

md5sum

8f69e04a65d6063121f6153e7230324c

Content MD5sum

012855f41b9bcb09adcaa61b31396212

File Size

34 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinA-PCR4-Rep1_S43_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GTCATCTA:TGCG

Average Read Length

Read Count

1762502

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM6871QVFB

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library