IGVFFI0694FRDB

released

validated

File Set

IGVFDS5686BCWV(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin0hrs-FF2-BinD-PCR2-Rep1_S22

md5sum

da266e2cbf46ded3db4f6e8c880fc007

Content MD5sum

e68c46717d565a7d942d7b88c4840320

File Size

11 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin0hrs-FF2-BinD-PCR2-Rep1_S22_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ATTATGTT:TTGA

Average Read Length

Read Count

515272

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0599CFOW

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library