Home

IGVFFI0679KMFP

released
validated
File Set
IGVFDS5699JBLV(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep2-FFrep4-BinC-PCRrep6_R2
md5sum
672fb9d5363f766c5ac34a8cfc6fa9d5
Content MD5sum
0fdf0589736af056ff4b0ad848852c14
File Size
6.4 MB
Submitted File Name
230807S022-THP1-P153-Amp_PPIF_promoter-BioRep2-FFrep4-BinC-PCRrep6_S366_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
R2
Index
GAACCTAG:TGAC
Average Read Length
150
Read Count
179544

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford