IGVFFI0662BBNP

released

validated

File Set

IGVFDS0508ERUQ(scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci)

Summary

R2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jay-shendure:CRISPRa_singlecell_K562_VPR_sequence_exprs_10Xlane2_seqlane2_R2

md5sum

88377ac059bdd0cdc268f7136b6751b1

Content MD5sum

8f89dfbd0ad6aa9a0546b2904bda47bf

File Size

6.6 GB

Submitted File Name

/net/shendure/vol10/www/content/members/CRISPRa_QTL_website/public/data/K562_data/sequencing_data/cellranger_mkfastq_output/outs/fastq_path/AAAHG2HHV/VPR_exprs_2/VPR_exprs_2_S4_L002_R2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NextSeq 2000

Sequencing Run

Flowcell ID

AAAHG2HHV

Lane

Sequencing Kit

NextSeq 1000/2000 P3 Reagent Kit

Illumina Read Type

Average Read Length

Read Count

145437085

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9542NUGS

InVitroSystem

K562

K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library