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IGVFFI0614TWOQ

released
validated
File Set
IGVFDS9381QGYL(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep1-FFrep1-BinC-PCRrep3_I1
md5sum
072a79c5e45844aac34007530f185db7
Content MD5sum
0ab9d99b595db4f5775e1dfa22cbfd28
File Size
2.4 MB
Submitted File Name
230523pPPIFEnformerTHP1-THP1-PE-pPPIF_Enformer-Amp-pPPIF-BioRep1-FFRep1_BinC-PCRrep3_S27_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I1
Index
CATAGCGA:CAGT
Average Read Length
8
Read Count
289473

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford