IGVFFI0584UVTC

released

validated

File Set

IGVFDS6541EVXL(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR4_Rep1_S46

md5sum

5cb4b56a207fc1f34c0e307c4d7ed590

Content MD5sum

303471339805278842a2e634386eb017

File Size

49 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinD-PCR4-Rep1_S46_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ATTGTCTG:TGCG

Average Read Length

Read Count

2517262

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9894YYOA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library