IGVFFI0578DOVK

released

validated

File Set

IGVFDS6678UHIV(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF2-BinD-PCR3_Rep1_S150

md5sum

d7670f491f6d5045e14c4c6640d2dc00

Content MD5sum

482a16a3e59117fbaf9ea53c68d1ea01

File Size

16 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF2-BinD-PCR3-Rep1_S150_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CCAGAGCT:TTGA

Average Read Length

Read Count

801574

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4390QYVS

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library