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IGVFFI0553UBWG

released
validated
File Set
IGVFDS5405RYGU(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep1-BinD-PCRrep7_R1
md5sum
723c54b7e7fccfbc4fff63dbcfa8fdf9
Content MD5sum
cf6ab1ff99be62f769289bc56bf3e511
File Size
6.1 MB
Submitted File Name
230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep1-BinD-PCRrep7_S133_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
R1
Index
TGCTCGAC:GTCA
Average Read Length
150
Read Count
190487

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford