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IGVFFI0547GPAW

released
validated
File Set
IGVFDS4761MJPK(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep2-FFrep1-BinD-PCRrep10_R2
md5sum
825974ccfd429413023a5c22371fa86c
Content MD5sum
915f9180c6299842fdb40b301ac40233
File Size
5.4 MB
Submitted File Name
230807S022-THP1-P153-Amp_PPIF_promoter-BioRep2-FFrep1-BinD-PCRrep10_S238_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
R2
Index
TCGCTAGA:ACTG
Average Read Length
150
Read Count
163108

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford