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IGVFFI0535KATF

released
validated
File Set
IGVFDS6761PEJX(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep3-BinB-PCRrep9_I1
md5sum
de3c8b83f1d0727fe59f24d1c61adaaa
Content MD5sum
c00140b3f8e32b61de58c2a27512f0a7
File Size
1.7 MB
Submitted File Name
230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep3-BinB-PCRrep9_S117_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I1
Index
CTAACTCG:CAGT
Average Read Length
8
Read Count
208034

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford