IGVFFI0513KQLE

released

validated

File Set

IGVFDS7080OXFZ(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF1-BinE-PCR1_S5

md5sum

4a9ac68e0c9a353780ecaecb9b99d214

Content MD5sum

33e8a57e6934c77cbd8903fd8a5f8f17

File Size

21 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF1-BinE-PCR1_S5_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AGCAATTC:CTCT

Average Read Length

Read Count

863994

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8090YJPJ

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library