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IGVFFI0503EQVR

released
validated
File Set
IGVFDS9665GYVE(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep4-BinC-PCRrep10_R2
md5sum
da9846da3297526784c91c5269ff7d39
Content MD5sum
14f5843a9f22656dac65f03dc7d75efe
File Size
6.8 MB
Submitted File Name
230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep4-BinC-PCRrep10_S178_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
R2
Index
GACCGTTG:CAGT
Average Read Length
150
Read Count
200005

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford