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IGVFFI0488NDBE

released
validated
File Set
IGVFDS9084GQXI(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep2-BinD-PCRrep11_I2
md5sum
b0bf18764a31454b8a15eee5034e7064
Content MD5sum
ad9f6c719140d74486b4b1b1c0101a16
File Size
2.2 MB
Submitted File Name
230807S022-THP1-P153-Amp_PPIF_promoter-BioRep1-FFrep2-BinD-PCRrep11_S95_I2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I2
Index
TGTAACTC:GTCA
Average Read Length
4
Read Count
280018

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford