{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI0485EDMQ/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI0485EDMQ",
"aliases": [
"jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep2-BinA-PCRrep8_R1"
],
"assay_titles": [
"Variant-EFFECTS"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "20a0355d240a23375d81980d3e1b3e30",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-13T10:13:04.775925+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/measurement-sets/IGVFDS5594RPYL/",
"accession": "IGVFDS5594RPYL",
"file_set_type": "experimental data",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM9903HPOM/",
"accession": "IGVFSM9903HPOM",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
"modality": "prime editing",
"status": "released",
"summary": "CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"status": "released",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"status": "released",
"summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus"
},
"file_size": 5887342,
"gene_list_for": [],
"href": "/sequence-files/IGVFFI0485EDMQ/@@download/IGVFFI0485EDMQ.fastq.gz",
"illumina_read_type": "R1",
"index": "ACTAAGAC:ACTG",
"input_file_for": [
"/tabular-files/IGVFFI8402CAPM/"
],
"integrated_in": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"loci_list_for": [],
"maximum_read_length": 150,
"md5sum": "e7c98027693314dfd079fd06ad9e4ade",
"mean_read_length": 150,
"minimum_read_length": 150,
"read_count": 198012,
"release_timestamp": "2023-12-21T00:48:25.103722+00:00",
"s3_uri": "s3://igvf-files/2023/12/13/624b8df2-1fb3-4f78-93df-bdad8b31cf16/IGVFFI0485EDMQ.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"sequencing_run": 1,
"status": "released",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_file_name": "230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep2-FFRep2_BinA-PCRrep8_S248_R1_001.fastq.gz",
"summary": "R1 reads from sequencing run 1",
"upload_status": "validated",
"uuid": "624b8df2-1fb3-4f78-93df-bdad8b31cf16"
}