IGVFFI0450VGJL

released

validated

File Set

IGVFDS9738NANI(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinA-PCR1_Rep1_S25

md5sum

e91a37ea84985c8cad9e73ec6f926920

Content MD5sum

e388e78ca99aafffdc54372d46198cd2

File Size

40 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinA-PCR1-Rep1_S25_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GAAGAAGT:TGCG

Average Read Length

Read Count

2064843

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM6871QVFB

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library