{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI0425XMWB/",
"@type": [
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"File",
"Item"
],
"accession": "IGVFFI0425XMWB",
"aliases": [
"jesse-engreitz:sequence_file-teloHAEC_2kG_library-NovaSeq1-scRNAseq_2kG_2PMDox1_1_S1_L001_R2_001.fastq.gz"
],
"assay_titles": [
"Perturb-seq"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "37c56a43fc4d311930683d7f7c4596e6",
"content_type": "reads",
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"creation_timestamp": "2023-12-20T22:48:25.370573+00:00",
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"file_format": "fastq",
"file_set": {
"@id": "/measurement-sets/IGVFDS7665VXNR/",
"accession": "IGVFDS7665VXNR",
"file_set_type": "experimental data",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM2673TSAF/",
"accession": "IGVFSM2673TSAF",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"status": "released",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"status": "released",
"term_name": "TeloHAEC"
}
],
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"summary": "TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"status": "preview",
"summary": "CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci"
},
"file_size": 13386192072,
"gene_list_for": [],
"href": "/sequence-files/IGVFFI0425XMWB/@@download/IGVFFI0425XMWB.fastq.gz",
"illumina_read_type": "R2",
"input_file_for": [],
"integrated_in": [],
"lab": {
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"title": "Jesse Engreitz, Stanford"
},
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"maximum_read_length": 60,
"md5sum": "631aeecd4c4fffc5aa6e8ed24e3c71f6",
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"schema_version": "14",
"seqspecs": [],
"sequencing_kit": "NovaSeq 6000 S4 Reagent Kit v1.5",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008637/",
"status": "released",
"term_name": "Illumina NovaSeq 6000"
},
"sequencing_run": 5,
"status": "preview",
"submitted_by": {
"@id": "/users/05fa0dc9-0e33-427c-9d1f-82393edc4a03/",
"title": "Helen Kang"
},
"submitted_file_name": "/seq/landerlabCE/Users/Helen/TeloHAEC_Perturb-seq_2kG/240210_IGVF_recombined_fastqs/scRNAseq_2kG_2PMDox1_1_L001_R2_001.fastq.gz",
"summary": "R2 reads from sequencing run 5",
"upload_status": "validated",
"uuid": "8a9ba3f3-178e-4c35-bed1-f9a5e4757d41"
}