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IGVFFI0411GLKE

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS5699EGGK(cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW427C_6_R2
md5sum
435f0f8e829afb10587f6193364c2401
Content MD5sum
344190afb7d37b2d9ae7434bedd372b1
File Size
5.1 GB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/HTO//LW427C_S21_L006_R2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles

Sequencing Details

Sequencing Platform
Illumina NovaSeq 6000
Sequencing Run
1
Flowcell ID
227C7WLT4
Lane
6
Sequencing Kit
NovaSeq X Series 25B Reagent Kit
Illumina Read Type
R2
Average Read Length
151
Read Count
76950029

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW427), male, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
1.0 GB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
768 MB
validated

Attribution

Award
HG011996
Principal Investigator(s)
Contact P.I.
Gary Hon
Lab
Gary Hon, UT