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IGVFFI0406DXRA

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS1156KOWV(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_CAAC-THP1-BioRep1-FFrep2-BinC-PCRrep2_I2
md5sum
45c50d74343788bf9ede0d07c3190ed9
Content MD5sum
1dbc566eb2f935c208aed0302b4a84ac
File Size
3.8 MB
Submitted File Name
240908-S029-5splice-THP1-5splicesite_C11-Amp_PPIF_5splicesite-BioRep1-FFrep2-BinC-PCRrep2_S841_I2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NovaSeq X
Sequencing Run
1
Illumina Read Type
I2
Index
CGCTATGT:CGCAGACG
Average Read Length
8
Read Count
468360

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford