IGVFFI0402AEUG

released
validated
File Set
IGVFDS3553DWNL(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinE-PCRrep2_I1
md5sum
e4f7926ef19133e7d20c12a143f7e43d
Content MD5sum
c25cf65f92cc1d7f941456bb14cba0d0
File Size
3.3 MB
Submitted File Name
220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S18-BioRep3-FFRep2-BinE-PCRrep2_S209_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Illumina Read Type
I1
Index
GTCGCCTT:ACTG
Average Read Length
8
Read Count
416014

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 80-90% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution