IGVFFI0393VYGB

released

validated

File Set

IGVFDS3611WVHW(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF1-BinE-PCR4-Rep1_S173

md5sum

1cc230ba43ff9cc6ba66cc836cb0089f

Content MD5sum

6618b983950920f90a7c38374991d114

File Size

30 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF1-BinE-PCR4-Rep1_S173_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CCTACCAT:ATTG

Average Read Length

Read Count

1370537

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4222TCYN

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library