IGVFFI0373YORO

released

validated

File Set

IGVFDS5675MHUS(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF2-BinD-PCR2-Rep1_S130

md5sum

aa755eb215618d02cff9955ed4e0833d

Content MD5sum

56d6f39a738288643d8fb5bb717a3187

File Size

23 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF2-BinD-PCR2-Rep1_S130_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GTCTGATG:CCAG

Average Read Length

Read Count

1077404

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8987EBZC

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library