IGVFFI0367KUMY

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS7494CPQT(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep3-BinD-PCRRep6_I2
md5sum
ca720400ab1bd4d34b91f3d39e0222d8
Content MD5sum
8a9adad39cf36ef71381976733e15272
File Size
2.0 MB
Submitted File Name
Jurkat_Stim-IL2RA_prom-BioRep2-FFRep3-PCRRep6-BinD_S144_I2_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Illumina Read Type
I2
Index
TTAATCAG:GGCA
Average Read Length
4
Read Count
254682

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
variant effects
variant effects
Jesse Engreitz, Stanford
5.9 KB
validated

Attribution