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IGVFFI0332PBFZ

released
validated
File Set
IGVFDS8929VNCP(gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jay-shendure:CRISPRa_singlecell_K562_VP64_sequence_CS1_10Xlane2_seqlane2_R1
External Resources
md5sum
81eae3cfabeeda902aa25593743d9543
Content MD5sum
e1f1117bc63b0e41c5a1524e3a5be568
File Size
855 MB
Submitted File Name
/net/shendure/vol10/www/content/members/CRISPRa_QTL_website/public/data/K562_data/sequencing_data/cellranger_mkfastq_output/outs/fastq_path/AAAHG2HHV/VP64_CS1_2/VP64_CS1_2_S6_L002_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAAHG2HHV
Lane
2
Sequencing Kit
NextSeq 1000/2000 P3 Reagent Kit
Illumina Read Type
R1
Average Read Length
28
Read Count
40359874

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library

Files Derived From This File

3 items
csv
differential gene expression quantifications
differential gene expression quantifications
Jay Shendure, UW
3.3 KB
validated
csv
differential gene expression quantifications
differential gene expression quantifications
Jay Shendure, UW
6.9 KB
validated
tar
sparse gene count matrix
cell by gene in sparse gene count matrix
Jay Shendure, UW
538 MB
validated

Attribution

Award
HG011966
Principal Investigator(s)
Contact P.I.
Jay Shendure
Lab
Jay Shendure, UW