IGVFFI0310CNHA

preview

validated

This object is in preview status. Metadata is subject to change until this object is released.

File Set

IGVFDS4537KNTB(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

I2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep2-BinB-PCRRep8_I2

md5sum

53eecc53acc02d77bdeb766ca14e6298

Content MD5sum

92766bcd0fb1069c1b3f43d20d8b1c27

File Size

2.0 MB

Submitted File Name

Jurkat_Stim-IL2RA_prom-BioRep2-FFRep2-PCRRep8-BinB_S186_I2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NovaSeq X

Sequencing Run

Illumina Read Type

Index

TAATGAAC:GGCA

Average Read Length

Read Count

265566

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3690RUXJ

InVitroSystem

Jurkat

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library