Home
Home
Search (⌘K or ⌃K)
Data
Methodology
Data Model
About
Help
Sign In
INDEXED
Home
/
SequenceFile
/
IGVFFI0296ZMGR
IGVFFI0296ZMGR
released
validated
Json view
File Set
IGVFDS0482NAKZ
(gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
md5sum
633d8efeadf1b15b49aa90257d5db42d
Content MD5sum
7473c7316ed516c8cf0807b39021e615
File Size
9.6 MB
Submitted File Name
/Desktop/NOS3/NOS3_CRISPRi_FlowFISH/231215S023-TeloHAEC-P219-Amp_NOS3-BioRep1-FFrep2-BinC-PCRrep1_S123_I2_001.fastq.gz
Sequencing Details
Sequencing Platform
Illumina MiSeq
Sequencing Run
1
Sequencing Kit
MiSeq Reagent Kit v2
Illumina Read Type
I2
Average Read Length
8
Read Count
1175684
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM7665HTFX
InVitroSystem
TeloHAEC
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford