IGVFFI0294ZOLF

released

validated

File Set

IGVFDS1575SRMY(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF4-BinE-PCR1_S41

md5sum

82dc8dfb8270397ce7731f8c00048f38

Content MD5sum

010f3cfc6895c70c39fb5005218e5103

File Size

9.5 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF4-BinE-PCR1_S41_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TCCTTGGT:CTCT

Average Read Length

Read Count

395497

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3377SBLH

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library