IGVFFI0242VPKF

released

validated

File Set

IGVFDS4252ROMJ(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR4_Rep2_S76

md5sum

03683d0e381cc7c944b0abcd3f129dc7

Content MD5sum

fab5a695e7401c4ec42b3eb19e734b22

File Size

32 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF1-BinC-PCR4-Rep2_S76_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TGTCGGAT:TCTC

Average Read Length

Read Count

1665943

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4577TQPO

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library