IGVFFI0237YEQF

released

validated

File Set

IGVFDS4010VNGF(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF2-BinB-PCR4-Rep1_S182

md5sum

5525e05ff0a3e5bb0c94d59055e822fb

Content MD5sum

75e90d32b4547f321033b5ab385d3048

File Size

22 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF2-BinB-PCR4-Rep1_S182_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ATTCTAGG:ATTG

Average Read Length

Read Count

1027426

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3569TFCR

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library