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IGVFFI0230PQCC

released
validated
File Set
IGVFDS5416ECKJ(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep2-FFrep2-BinC-PCRrep2_R2
md5sum
d2a0e967caf8bbc2568bc5c52d4911e9
Content MD5sum
bd2d4735d6791af785e867d49cc3fca9
File Size
76 MB
Submitted File Name
220228pPPIF-FFSample-8-D21-pPPIF-Amp-pPPIF-2-2-2-BinC_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
R2
Index
GACCAGGA:TGAC
Average Read Length
150
Read Count
1949301

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 35-45% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford