IGVFFI0229FWHC

preview

validated

This object is in preview status. Metadata is subject to change until this object is released.

File Set

IGVFDS2226SPYF(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

I2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep3-BinD-PCRRep1_I2

md5sum

1757b40f20895c400e13d476a2470ed9

Content MD5sum

4a613639144b4c728ae0b9896a35578e

File Size

1.4 MB

Submitted File Name

Jurkat_Stim-IL2RA_prom-BioRep1-FFRep3-PCRRep1-BinD_S12_I2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NovaSeq X

Sequencing Run

Illumina Read Type

Index

AGGTCGCA:TTGT

Average Read Length

Read Count

180973

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM5709XAIS

InVitroSystem

Jurkat

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library