IGVFFI0227JJOM

released

validated

File Set

IGVFDS5616WTVS(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin6hrs-FF4-BinF-PCR1_S90

md5sum

b682e2ca65f2495a3017b1e2225692c9

Content MD5sum

3fb44bcd923d559b63f13dc1ad7f3954

File Size

4.9 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin6hrs-FF4-BinF-PCR1_S90_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CTGCGGAT:CTCT

Average Read Length

Read Count

202101

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1655HPGH

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library