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IGVFFI0178FLZC

released
validated
File Set
IGVFDS2708HTXW(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep1-BinA-PCRrep3_I2
md5sum
4a0aff9629b0ffee6498360ecb1c38c5
Content MD5sum
aea9bfc197d1fe9773bc875fe1fedb6c
File Size
909 KB
Submitted File Name
230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep1-BinA-PCRrep3_S3_I2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I2
Index
GCACACGA:CAGT
Average Read Length
4
Read Count
105273

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford