IGVFFI0132JLWC

released

validated

File Set

IGVFDS5581ABLR(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF1-BinE-PCR3_Rep2_S72

md5sum

1b7286eb41d7fe0aef6d5c7db1ebb30c

Content MD5sum

70dd55ee3b7b9c035e8e0130973648d6

File Size

10 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF1-BinE-PCR3-Rep2_S72_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GCACACGA:TCTC

Average Read Length

Read Count

516220

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7084WNRY

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library