IGVFFI0124EDRT

preview

validated

This object is in preview status. Metadata is subject to change until this object is released.

File Set

IGVFDS1434CFNM(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

I1 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep2-BinD-PCRRep2_I1

md5sum

f19aa8246d811c28b541eb7610a4bac2

Content MD5sum

bd0c554433d42fc39411e0608fcf581f

File Size

1.6 MB

Submitted File Name

Jurkat_Stim-IL2RA_prom-BioRep1-FFRep2-PCRRep2-BinD_S32_I1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NovaSeq X

Sequencing Run

Illumina Read Type

Index

GCCGTCGA:TTGT

Average Read Length

Read Count

192660

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9448WLVQ

InVitroSystem

Jurkat

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library