Home

IGVFFI0117NBZL

released
validated
File Set
IGVFDS4371VPAL(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep1-FFrep2-BinD-PCRrep2_I2
md5sum
2dd56f85b5230f170a3f35d328c0915d
Content MD5sum
2fe535c461e572a028549f231c849c9c
File Size
14 MB
Submitted File Name
220228pPPIF-FFSample-3-D21-pPPIF-Amp-pPPIF-1-2-2-BinD_I2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I2
Index
AGCATGGA:TGAC
Average Read Length
4
Read Count
1956760

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 55-65% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford